Vitamin D, 25-Hydroxy levels: what's normal, high and low
Your body's vitamin D status. Influenced by sun exposure, diet, supplements, and absorption.
Medically reviewed by Youmna DiStefano, MD · Last reviewed September 2026
The short answer
Vitamin D, 25-Hydroxy is measured with a standard blood draw; no fasting is needed. A typical reference range is 30.0 – 100 ng/mL. At TestWell it's $41.99 all-in as Vitamin D, 25-Hydroxy, results in 1–3 business days, drawn at Quest or Labcorp. An out-of-range result is a prompt to look closer, not a diagnosis.
- Units
- ng/mL
- Sample
- Blood draw
- Fasting
- Not required
- Results
- 1–3 business days
- Price
- $41.99
- Labs
- Quest or Labcorp · BioReference in NY/NJ
Vitamin D, 25-Hydroxy Reference Range
These are the typical adult reference ranges used by major US labs. Your report will include the specific range used by the lab that processed your sample.
| Population | Range |
|---|---|
| age 18+ | 30.0 – 100 ng/mL |
Reference ranges are educational. Always interpret your result with the specific range printed on your lab report and discuss anything outside the range with a clinician.
What your Vitamin D, 25-Hydroxy result may mean
Usually reflects supplement intake. Very high levels (>100 ng/mL) can occur with over-supplementation.
What a high Vitamin D, 25-Hydroxy means: causes, symptoms, next steps →Common in people with limited sun exposure, darker skin, obesity, or absorption issues. Supplementation is often used to correct it.
What a low Vitamin D, 25-Hydroxy means: causes, symptoms, next steps →Educational only — not a diagnosis. Many factors can influence a single result. Discuss anything that concerns you with a healthcare provider.
How do you get a Vitamin D, 25-Hydroxy test?
Order online, a licensed physician reviews the requisition, and you walk into a nearby lab for the draw. Vitamin D, 25-Hydroxy is available on its own or inside these panels — panels cost less per marker when you want the fuller picture.
| Option | Includes | Price |
|---|---|---|
| Vitamin D, 25-Hydroxy | This marker only | $41.99 |
| Basic Vitamin Deficiency Panel | 3 tests | $89.99 |
| Post-COVID Recovery Panel | 7 tests | $99.99 |
| Metabolism & Weight Loss Panel | 8 tests | $119.99 |
| Fitness & Performance Panel | 8 tests | $119.99 |
Quest vs. Labcorp for Vitamin D, 25-Hydroxy: which lab should you pick?
Both are CLIA-certified national labs and the result is clinically equivalent, so pick whichever location is closer. Your report will print that lab's own reference range, which can differ slightly from the table above.
| Lab | Available | Price |
|---|---|---|
| Quest Diagnostics | Yes | $41.99 |
| Labcorp | Yes | $41.99 |
| BioReference (NY & NJ) | Yes | $44.99 |
Need help choosing a location? Use the lab finder to see draw sites near your ZIP.
How often should you retest Vitamin D, 25-Hydroxy?
If you're correcting a low level, retest about 3 months after starting supplementation — that's roughly how long 25-hydroxyvitamin D takes to plateau on a steady dose, per the Endocrine Society's deficiency guideline. Once in range, many people recheck annually, often in late winter when levels are lowest.
General guidance from the sources listed below — your clinician may set a different schedule based on your history and results.
Related Vitamins & Nutrients biomarkers
Markers commonly tested alongside Vitamin D, 25-Hydroxy.
Frequently asked
Usually reflects supplement intake. Very high levels (>100 ng/mL) can occur with over-supplementation. A single result above the reference range is a prompt to look closer with a clinician — often a repeat test and related markers — not a diagnosis on its own.
Ready to test your vitamin d, 25-hydroxy?
Order in two minutes, get physician-approved, and visit a lab the same day. Starts at $41.99.