Also known as: 25-Hydroxy Vitamin D, Vitamin D 25-OH, Vitamin D Test
The short answer
The definitive vitamin D test — an estimated 42% of American adults are deficient, measured from a standard blood draw. At TestWell it costs $41.99 plus a $6 lab fee, needs no fasting, and results post in 1–3 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Quest or Labcorp.
Hospital Lab
$150
cash pay
Other Labs
$75
+ lab fees
TestWell
$41.99
+ $6 lab fee
The 25-Hydroxy Vitamin D test is the standard way to measure vitamin D status. Vitamin D is essential for bone health, immune function, mood regulation, and muscle strength. An estimated 42% of American adults are deficient, with higher rates among those with darker skin, limited sun exposure, or who live in northern climates. Optimal levels are generally considered to be 30–50 ng/mL.
Measures 25-Hydroxy Vitamin D, the storage form. The standard test for vitamin D deficiency, which is implicated in bone health, immune function, and mood.
Anyone with fatigue, low mood (especially in winter), bone or muscle pain, frequent illness, or limited sun exposure. The single most commonly deficient nutrient we see — an estimated 42% of US adults are deficient.
Sample Type
Blood draw
Fasting
Not required
Results In
1–3 business days
Performed At
Quest or Labcorp
Your Vitamin D, 25-Hydroxy results will include values for each of the 1 biomarker tested, along with reference ranges. Results are reviewed by a licensed physician before being released to your secure portal.
If any values fall outside the normal range, your report will include guidance on what that may mean and recommended next steps. You'll receive an email notification when your results are ready, typically within 1–3 business days.
Typical reference range layout
Lab reports show where your value sits relative to established reference intervals—your physician interprets this in context for you.
No fasting required. No special preparation needed.
The short answer
Worth it if you have symptoms or risk factors, but not something everyone needs annually. It is the right call for fatigue, bone or muscle aches, limited sun exposure, or before/while supplementing - so you dose based on a number, not a guess.
We would rather you order the right test than the most expensive one.
Vitamin D (25-hydroxy) is a venous draw at Quest or Labcorp, or an at-home mobile draw where available. Some fingerstick kits exist, but the venous lab version is the reference standard used for diagnosis and dosing. No fasting required.
The markers that matter most and what an out-of-range value can mean. Reference ranges vary by lab, age and sex.
| Marker | Typical range | What it means |
|---|---|---|
| 25-hydroxy vitamin D | 30-100 ng/mL sufficient | Under 20 ng/mL is deficient and 20-29 is insufficient; very high levels (usually over 100) come from over-supplementing and can be harmful. |
This section medically reviewed by Youmna DiStefano, MD — updated June 2026. General health information, not medical advice; interpret results with a qualified clinician.
A Vitamin D, 25-Hydroxy test costs $41.99 at TestWell — $47.99 all-in when ordered alone, because the $6 lab processing fee is charged once per order, with no draw fee or physician fee added. Ordered directly from the labs' own consumer sites, the same test lists at $75.00 at Quest Health and $99.00 at Labcorp OnDemand (list prices checked Sep 12, 2026). Hospital outpatient labs typically bill around $150.00 self-pay for the same test, and often more. With insurance, what you pay depends on your deductible and your plan's negotiated rate; TestWell's price is fixed before the draw and is HSA/FSA-eligible.
| Where you order | Price | One test, all-in | Notes |
|---|---|---|---|
| TestWell (drawn at Quest or Labcorp) | $41.99 | $47.99 | Includes the one $6 lab processing fee, charged once per order |
| Quest Health (Quest Diagnostics' own site) | $75.00 | $75.00 | list price checked Sep 12, 2026 |
| Labcorp OnDemand (Labcorp's own site) | $99.00 | $99.00 | list price checked Sep 12, 2026 |
| Hospital outpatient lab, self-pay (typical) | $150.00 | $150.00 | Reference figure; hospital charges vary widely by facility and are often higher |
36% less than the lower lab-direct list price, for the same draw at the same lab. Lab test codes: Quest test code 91735 · Labcorp test no. 081950 · BioReference code 0286-5. Competitor prices are read from the linked public pages and re-checked regularly; the labs' sites may add taxes or change prices without notice.
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TestWell Price
$41.99
+ $6 lab processing fee at checkout
No insurance needed · HIPAA compliant
Better value: the Basic Vitamin Deficiency Panel
Save $10.98This test is part of the Basic Vitamin Deficiency Panel — 3 tests · 3 biomarkers for $89.99, $10.98 less than à la carte.
$89.99$100.97
3 tests · 3 biomarkers
Frequently ordered together
Customers who ordered Vitamin D, 25-Hydroxy also added these for a more complete health picture.
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Vitamin D, 25-Hydroxy
$41.99