Also known as: 25-Hydroxy Vitamin D, Vitamin D 25-OH, Vitamin D Test
Hospital Lab
$150
cash pay
Other Labs
$75
+ lab fees
TestWell
$41.99
+ $6 lab fee
The 25-Hydroxy Vitamin D test is the most accurate way to measure vitamin D status. Vitamin D is essential for bone health, immune function, mood regulation, and muscle strength. An estimated 42% of American adults are deficient, with higher rates among those with darker skin, limited sun exposure, or who live in northern climates. Optimal levels are generally considered to be 30–50 ng/mL.
Measures 25-Hydroxy Vitamin D, the storage form. The standard test for vitamin D deficiency, which is implicated in bone health, immune function, and mood.
Anyone with fatigue, low mood (especially in winter), bone or muscle pain, frequent illness, or limited sun exposure. The single most commonly deficient nutrient we see — an estimated 42% of US adults are deficient.
Sample Type
Blood draw
Fasting
Not required
Results In
1–3 business days
Performed At
Quest or Labcorp
Your Vitamin D, 25-Hydroxy results will include values for each of the 1 biomarker tested, along with reference ranges. Results are reviewed by a licensed physician before being released to your secure portal.
If any values fall outside the normal range, your report will include guidance on what that may mean and recommended next steps. You'll receive an email notification when your results are ready, typically within 1–3 business days.
Typical reference range layout
Lab reports show where your value sits relative to established reference intervals—your physician interprets this in context for you.
Your Vitamin D, 25-Hydroxy results will include values for each of the 1 biomarker tested, along with reference ranges. Results are reviewed by a licensed physician before being released to your secure portal.
If any values fall outside the normal range, your report will include guidance on what that may mean and recommended next steps. You'll receive an email notification when your results are ready, typically within 1–3 business days.
Typical reference range layout
Lab reports show where your value sits relative to established reference intervals—your physician interprets this in context for you.
No fasting required. No special preparation needed.
No fasting required. No special preparation needed.
Is a Vitamin D, 25-Hydroxy worth it?
Our guide: at-home vs lab, cost, and how to read your results.
TestWell Price
$41.99
+ $6 lab processing fee at checkout
No insurance needed · HIPAA compliant
Better value: the Basic Vitamin Deficiency Panel
Save $10.98This test is part of the Basic Vitamin Deficiency Panel — 3 tests · 3 biomarkers for $89.99, $10.98 less than à la carte.
$89.99$100.97
3 tests · 3 biomarkers
Frequently ordered together
Customers who ordered Vitamin D, 25-Hydroxy also added these for a more complete health picture.
Not sure where to start?
Vitamin D, 25-Hydroxy
$41.99