Also known as: 25-Hydroxy Vitamin D, Vitamin D 25-OH, Vitamin D Test
On your lab requisition: Quest “Cardio IQ Vitamin D, 25-Hydroxy” (test code 91735)
The short answer
The definitive vitamin D test — an estimated 42% of American adults are deficient, measured from a standard blood draw. At TestWell it costs $41.99 plus a $6 lab processing fee, needs no fasting, and results post in 1–3 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Quest or Labcorp.
Hospital Lab
$150
cash pay
Other Labs
$75
+ lab fees
TestWell
$41.99
+ $6 lab processing fee
The 25-Hydroxy Vitamin D test is the standard way to measure vitamin D status. Vitamin D is essential for bone health, immune function, mood regulation, and muscle strength. An estimated 42% of American adults are deficient, with higher rates among those with darker skin, limited sun exposure, or who live in northern climates. Optimal levels are generally considered to be 30–50 ng/mL.
Measures 25-Hydroxy Vitamin D, the storage form. The standard test for vitamin D deficiency, which is implicated in bone health, immune function, and mood.
Anyone with fatigue, low mood (especially in winter), bone or muscle pain, frequent illness, or limited sun exposure. The single most commonly deficient nutrient we see — an estimated 42% of US adults are deficient.
Sample Type
Blood draw
Fasting
Not required
Results In
1–3 business days
Performed At
Quest or Labcorp
Your Vitamin D, 25-Hydroxy results will include values for each of the 1 biomarker tested, along with reference ranges. Results are reviewed by a licensed physician before being released to your secure portal.
If any values fall outside the normal range, your report will include guidance on what that may mean and recommended next steps. You'll receive an email notification when your results are ready, typically within 1–3 business days.
Typical reference range layout
Lab reports show where your value sits relative to established reference intervals—your physician interprets this in context for you. Already have results? Analyze them with your AI assistant against our physician-reviewed ranges.
No fasting required. No special preparation needed.
What can throw off this result
Physician-reviewed “Before your test” pages — what each factor does, for how long, and what to do about it.
The short answer
Worth it if you have symptoms or risk factors, but not something everyone needs annually. It is the right call for fatigue, bone or muscle aches, limited sun exposure, or before/while supplementing - so you dose based on a number, not a guess.
We would rather you order the right test than the most expensive one.
Vitamin D (25-hydroxy) is a venous draw at Quest or Labcorp, or an at-home mobile draw where available. Some fingerstick kits exist, but the venous lab version is the reference standard used for diagnosis and dosing. No fasting required.
The markers that matter most and what an out-of-range value can mean. Reference ranges vary by lab, age and sex.
| Marker | Typical range | What it means |
|---|---|---|
| 25-hydroxy vitamin D | 30-100 ng/mL sufficient | Under 20 ng/mL is deficient and 20-29 is insufficient; very high levels (usually over 100) come from over-supplementing and can be harmful. |
This section medically reviewed by Youmna DiStefano, MD — updated June 2026. General health information, not medical advice; interpret results with a qualified clinician.
A 25-Hydroxy Vitamin D test costs $41.99 at TestWell — $47.99 all-in when ordered alone, because the $6 lab processing fee is charged once per order, with no draw fee or physician fee added. Ordered directly from the labs' own consumer sites, the same test lists at $75.00 at Quest Health and $99.00 at Labcorp OnDemand (list prices checked Sep 12, 2026). Hospital outpatient labs typically bill around $150.00 self-pay for the same test, and often more. With insurance, what you pay depends on your deductible and your plan's negotiated rate; TestWell's price is fixed before the draw and is HSA/FSA-eligible. Full cost breakdown →
| Where you order | Price | One test, all-in | Notes |
|---|---|---|---|
| TestWell (drawn at Quest or Labcorp) | $41.99 | $47.99 | Includes the one $6 lab processing fee, charged once per order |
| TestWell (drawn at BioReference — New York & New Jersey) | $44.99 | $50.99 | New York and New Jersey orders route to BioReference; Quest Health and Labcorp OnDemand don't take self-ordered draws in those states |
| Quest Health (Quest Diagnostics' own site) | $75.00 | $75.00 | list price checked Sep 12, 2026 |
| Labcorp OnDemand (Labcorp's own site) | $99.00 | $99.00 | list price checked Sep 12, 2026 |
| Hospital outpatient lab, self-pay (typical) | $150.00 | $150.00 | Reference figure; hospital charges vary widely by facility and are often higher |
36% less than the lower lab-direct list price, for the same draw at the same lab. Lab test codes: Quest test code 91735 · Labcorp test no. 081950 · BioReference code 0286-5. Competitor prices are read from the linked public pages and re-checked regularly; the labs' sites may add taxes or change prices without notice.
Compare more: Quest Diagnostics price list · Labcorp price list · Quest vs Labcorp · Lab test price checker
Most labs report deficient under 20 ng/mL, insufficient 20-30 ng/mL, sufficient 30-100 ng/mL. The Endocrine Society recommends 40-60 ng/mL for optimal immune and bone health. Above 100 ng/mL may indicate over-supplementation.
25-Hydroxy (this test) measures storage and is the standard for diagnosing deficiency. 1,25-Dihydroxy (the active form) is only ordered when investigating specific conditions like sarcoidosis, kidney disease, or unexplained hypercalcemia.
Absorption varies hugely between people — same 2000 IU dose can produce levels ranging from 20 to 80 ng/mL depending on body weight, gut health, and genetics. The only way to know if your dose is working is to measure. Re-test 8-12 weeks after starting or changing a dose.
On TestWell, the 25-Hydroxy Vitamin D test is $41.99. Quest Direct lists the same test at $75, walk-in hospital labs typically bill $130-150 without insurance. Same CLIA-certified labs, same biomarker — TestWell just removes the markup.
The CPT code for 25-Hydroxy Vitamin D is 82306. The active form (1,25-Dihydroxy) is billed as 82652 and is rarely ordered without specific clinical indication. Cash-pay patients don't need a CPT code to order through TestWell.
No fasting required. Vitamin D levels are stable throughout the day and unaffected by recent meals. You can draw any time.
Once a year if your levels are stable in range. Every 3 months if you're correcting a deficiency, adjusting a supplement dose, or recovering from a major illness.
The 25-hydroxy vitamin D test is the correct one for checking your status; it reflects your body's stores. The 1,25-dihydroxy test is a specialty test for specific kidney and calcium disorders and is not used for routine deficiency screening.
Vitamin D levels change slowly. Wait about 8-12 weeks after starting or changing a supplement before retesting, which gives your 25-hydroxy level time to reach a new steady state.
Yes - you can order online without a doctor and get a venous draw at Quest or Labcorp, or an at-home mobile draw where available. That venous sample is the same reference-standard test a physician would order, unlike consumer fingerstick kits.
A vitamin D 25-hydroxy test costs $41.99 out of pocket at TestWell ($47.99 all-in with the $6 per-order lab processing fee) — it is sold here as the Vitamin D, 25-Hydroxy, with no doctor's visit or insurance required. Ordered directly from the labs' own sites, the same test lists at $75.00 at Quest Health and $99.00 at Labcorp OnDemand (checked September 2026). Results in 1–3 business days.
Ordered directly, a 25-Hydroxy Vitamin D test lists at $75.00 at Quest Health (Quest Diagnostics' own site) and $99.00 at Labcorp OnDemand (prices checked September 2026). TestWell orders the same test at the same Quest or Labcorp patient service centers for $41.99 — $47.99 all-in with the $6 per-order lab processing fee — paid out of pocket at checkout with no insurance, doctor's visit or draw fee; HSA and FSA cards are accepted.
Biotin supplements, vitamin D supplements and smoking and nicotine can change a 25-Hydroxy Vitamin D test result without anything being wrong. Biotin supplements can falsely raise 25-Hydroxy Vitamin D — stop biotin ≥72 hours before the draw. Vitamin D supplements can interfere with 25-Hydroxy Vitamin D — keep your normal routine; note dose for interpretation. Smoking and nicotine can lower 25-Hydroxy Vitamin D — deficiency is common in smokers and worth correcting. Each factor is covered on our physician-reviewed "Before your test" pages; when one applies, the fix is usually to remove it and retest.
Order the 25-Hydroxy Vitamin D test online from TestWell for $41.99 (plus a $6 per-order lab processing fee) — no doctor's visit, referral or insurance needed. A licensed physician authorizes the order, then you walk into any Quest or Labcorp patient service center near you for the draw (the lab locator on our site lists the closest ones by ZIP code), and results arrive in 1–3 business days.
The 25-Hydroxy Vitamin D test is Quest Diagnostics test code 91735 ("Cardio IQ Vitamin D, 25-Hydroxy"), Labcorp test number 081950, BioReference code 0286-5 (CPT 82306). TestWell orders it under those codes for $41.99 at Quest, Labcorp or BioReference — no doctor's visit or insurance required, results in 1–3 business days. Codes verified against the labs' catalogs in 2026.
Medically reviewed by Youmna DiStefano, MD · Last reviewed September 2026
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TestWell Price
$41.99
+ $6 lab processing fee at checkout
No insurance needed · HIPAA compliant
Better value: the Basic Vitamin Deficiency Panel
Save $10.98This test is part of the Basic Vitamin Deficiency Panel — 3 tests · 3 biomarkers for $89.99, $10.98 less than à la carte.
$89.99$100.97
3 tests · 3 biomarkers
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Vitamin D, 25-Hydroxy
$41.99