The short answer
Measures the active form of Vitamin D — essential for evaluating kidney disease, calcium disorders, and sarcoidosis, measured from a standard blood draw. At TestWell it costs $59.99 plus a $6 lab fee, needs no fasting, and results post in 3–5 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Labcorp.
Hospital Lab
$250
cash pay
Other Labs
$99
+ lab fees
TestWell
$59.99
+ $6 lab fee
Vitamin D 1,25-Dihydroxy (calcitriol) is the biologically active form of vitamin D, produced primarily in the kidneys. Unlike the more commonly tested 25-Hydroxy form (which reflects vitamin D stores), calcitriol reflects how much active vitamin D your body is actually using. This test is essential for evaluating patients with kidney disease (where conversion to active vitamin D is impaired), hypercalcemia, hypocalcemia, sarcoidosis, and other granulomatous diseases. It is also useful when 25-Hydroxy vitamin D levels are normal but symptoms of vitamin D-related disease persist. The two forms of vitamin D should be interpreted together for a complete picture.
Vitamin and nutrient testing identifies deficiencies that can drive fatigue, immune dysfunction, neurological symptoms, and long-term disease risk — and confirms that supplementation is working.
Anyone with restrictive diets (vegan, low-fat, low-carb), malabsorption conditions, fatigue, hair loss, mood changes, or currently supplementing and wanting to verify dosing.
Sample Type
Blood draw
Fasting
Not required
Results In
3–5 business days
Performed At
Labcorp
1 biomarker tested
Your Vitamin D, 1,25-Dihydroxy (Calcitriol) results will include values for each of the 1 biomarker tested, along with reference ranges. Results are reviewed by a licensed physician before being released to your secure portal.
If any values fall outside the normal range, your report will include guidance on what that may mean and recommended next steps. You'll receive an email notification when your results are ready, typically within 3–5 business days.
Typical reference range layout
Lab reports show where your value sits relative to established reference intervals—your physician interprets this in context for you.
No fasting required. Continue all medications and supplements as normal unless directed otherwise by your provider.
A Vitamin D, 1,25-Dihydroxy (Calcitriol) test costs $59.99 at TestWell — $65.99 all-in when ordered alone, because the $6 lab processing fee is charged once per order, with no draw fee or physician fee added. Hospital outpatient labs typically bill around $250.00 self-pay for the same test, and often more. With insurance, what you pay depends on your deductible and your plan's negotiated rate; TestWell's price is fixed before the draw and is HSA/FSA-eligible.
| Where you order | Price | One test, all-in | Notes |
|---|---|---|---|
| TestWell (drawn at Quest or Labcorp) | $59.99 | $65.99 | Includes the one $6 lab processing fee, charged once per order |
| Hospital outpatient lab, self-pay (typical) | $250.00 | $250.00 | Reference figure; hospital charges vary widely by facility and are often higher |
Lab test codes: Quest test code 16761 · Labcorp test no. 081091. Competitor prices are read from the linked public pages and re-checked regularly; the labs' sites may add taxes or change prices without notice.
Compare more: Quest Diagnostics price list · Labcorp price list · Quest vs Labcorp · Lab test price checker
Medically reviewed by Youmna DiStefano, MD · Last reviewed September 2026
TestWell Price
$59.99
+ $6 lab processing fee at checkout
No insurance needed · HIPAA compliant
Frequently ordered together
Customers who ordered Vitamin D, 1,25-Dihydroxy (Calcitriol) also added these for a more complete health picture.
Not sure where to start?
Vitamin D, 1,25-Dihydroxy (Calcitriol)
$59.99