Quest Diagnostics at 5463 Gateway Circle, Suite 102, Orlando, FL
Quest Diagnostics patient service center, 7 miles from downtown Orlando. Open Mon–Fri 6:00AM – 3:00PM · Sat 6:00AM – 12:00PM · Sun closed. Order your tests on TestWell, choose Quest at checkout, and walk in with the emailed requisition and a photo ID — no doctor's visit, referral or insurance; results post to your portal in 1–3 business days. Address, phone and hours were read from the lab's listing on October 10, 2026.
- Address
- 5463 Gateway Circle, Suite 102, Orlando, FL 32812
- Phone
- 407-726-6344
- Appointment
- Walk-ins at most sites · book on Quest's site to skip the wait
Hours at Quest Diagnostics, 5463 Gateway Circle
As listed by the lab on October 10, 2026; confirm before a holiday or Saturday visit.
| Monday | 6:00AM – 3:00PM |
|---|---|
| Tuesday | 6:00AM – 3:00PM |
| Wednesday | 6:00AM – 3:00PM |
| Thursday | 6:00AM – 3:00PM |
| Friday | 6:00AM – 3:00PM |
| Saturday | 6:00AM – 12:00PM |
| Sunday | Closed |
How to get a blood test at this Quest Diagnostics location
- Choose your tests and check out, picking Quest as your lab. A licensed physician authorizes the order, usually within minutes, and the requisition is emailed to you — one $6 lab processing fee per order, nothing to pay here.
- Come to 5463 Gateway Circle, Suite 102 during the hours above with the requisition (on your phone is fine) and a photo ID. Fasting tests are easiest first thing in the morning.
- Results post to your TestWell portal in 1–3 business days with reference ranges and a physician review; download the report to share with your own doctor.
Popular tests drawn at Quest Diagnostics in Orlando — prices at Quest
The tests Florida customers order most, September 2025 – September 2026, priced for a Quest draw. Plus one $6 fee per order.
| Test | Price at Quest | Share of orders |
|---|---|---|
| Comprehensive Metabolic Panel | $22.99 | 11.7% |
| CBC with Differential | $21.99 | 8.6% |
| Basic Metabolic Panel | $20.99 | 8% |
| Peptide Therapy Monitoring Panel | $259.99 | 7.4% |
| IGF-1 (Insulin-like Growth Factor) | $37.99 | 6.2% |
| TSH + Free T4 | $27.99 | 6.2% |
Other locations near 5463 Gateway Circle, Suite 102
- Labcorp1.8 mi
5425 S Semoran Blvd, Orlando, FL 32822
Weekdays 6:30AM – 3:00PM · Sat 7:00AM – 12:00PM
- Labcorp4.7 mi
4401 S Orange Ave, Ste 110, Orlando, FL 32806
Weekdays 6:30AM – 3:00PM
7780 Lake Underhill Rd, Orlando, FL 32822
Weekdays 5:00AM – 3:30PM · Sat 6:00AM – 12:00PM
- Quest Diagnostics6.1 mi
1700 Lucerne Ter, Orlando, FL 32806
Weekdays 6:00AM – 3:00PM · Sat 6:30AM – 12:30PM
- Labcorp at Walgreens7.1 mi
2420 E Colonial Dr, Orlando, FL 32803
Weekdays 7:00AM – 12:00PM & 12:30PM – 3:00PM
- Quest Diagnostics8.1 mi
1430 N Mills Ave, Unit 140, Orlando, FL 32803
Weekdays 6:30AM – 3:30PM · Sat 6:30AM – 12:30PM
Frequently asked questions
- What are the hours at Quest Diagnostics 5463 Gateway Circle in Orlando?
- Mon–Fri 6:00AM – 3:00PM · Sat 6:00AM – 12:00PM · Sun closed (as listed by the lab on 2026-10-10). Hours change around holidays — confirm before a Saturday or early-morning visit at 407-726-6344.
- Is Quest Diagnostics 5463 Gateway Circle open on Saturday?
- Yes — Saturday hours are 6:00AM – 12:00PM as of 2026-10-10. Saturday mornings fill up; an appointment on Quest Diagnostics's site shortens the wait.
- Do I need an appointment at Quest Diagnostics 5463 Gateway Circle?
- Most Quest Diagnostics patient service centers take walk-ins, and Quest Diagnostics lets you book a time slot on its site, which shortens the wait at busy locations. Bring a photo ID and your requisition — through TestWell it arrives by email within minutes of the physician's authorization, and it is accepted here as written.
- Can I get blood work at this Quest Diagnostics location without a doctor?
- Yes, with a requisition: Quest Diagnostics draws what a requisition orders but does not write orders. Order on TestWell — a licensed physician in our network authorizes it as part of the price, with one $6 lab processing fee per order — choose Quest at checkout, and walk in here. No insurance, referral or office visit; HSA and FSA cards are accepted.
- How much does a blood test cost at Quest Diagnostics in Orlando?
- Through TestWell the price is the test's listed price plus one $6 fee per order, with nothing to pay at the draw. For example, Comprehensive Metabolic Panel is $22.99, CBC with Differential is $21.99, Basic Metabolic Panel is $20.99 drawn at Quest Diagnostics. Ordered through a doctor and billed to insurance, your share depends on your plan.
- What is the phone number for Quest Diagnostics 5463 Gateway Circle, Orlando?
- 407-726-6344. Phone and hours were read from the lab's listing on 2026-10-10.