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What blood tests check for causes of unexplained weight gain?

Short answer

When weight climbs without a change in eating or activity, the testable causes are an underactive thyroid, insulin resistance and cortisol excess. The workup is TSH, hemoglobin A1c with fasting insulin and glucose, a lipid panel, and morning cortisol; PCOS markers are added for women with irregular cycles.

Medically reviewed by Youmna DiStefano, MD · Last reviewed September 2026

When should you see a clinician instead of ordering a test?

  • Rapid weight gain with swelling of the legs, shortness of breath or lying-flat breathlessness — seek care promptly
  • Weight gain with a rounded face, purple stretch marks, easy bruising and high blood pressure — see a clinician

Why these tests for unexplained weight gain?

Thyroid hormone sets energy expenditure, so hypothyroidism is the first thing to exclude. Insulin resistance — high fasting insulin with normal or rising glucose — makes weight gain and hunger worse years before diabetes appears, and A1c shows where blood sugar has been over three months.

Cortisol excess is rare but produces central weight gain, and morning cortisol is the screen. For women, weight gain with irregular periods or acne points to a PCOS workup. Medications (steroids, some antidepressants and antipsychotics) are a common non-lab cause worth raising with a clinician.

Which conditions do these tests help evaluate?

  • Unexplained Weight Gain Thyroid, insulin, cortisol and metabolic markers behind stubborn weight changes.
  • Thyroid Problems TSH, Free T4/T3 and antibodies to evaluate an under- or over-active thyroid.
  • Diabetes & Blood Sugar A1c, fasting glucose and insulin to screen for prediabetes and diabetes.
  • PCOS Androgens, LH/FSH, insulin and thyroid markers to investigate PCOS.

Which tests, and what do they cost?

Prices are cash-pay; every order adds one $6 lab processing fee. Physician authorization is included; no insurance or referral needed.

Test or panelTypePriceFastingResults
Metabolism & Weight Loss PanelSaves $105.93 vs ordering the tests separatelyPanel · 8 tests$119.99Yes2–5 business days
Complete Thyroid PanelSaves $10.97 vs ordering the tests separatelyPanel · 4 tests$109.99No3–7 business days
Pre-Diabetes PanelSaves $34.96 vs ordering the tests separatelyPanel · 5 tests$89.99Yes2–5 business days
TSH (Thyroid Stimulating Hormone)Test$26.99No1–3 business days
Hemoglobin A1cTest$21.99No2–4 business days
Insulin, FastingTest$22.99Yes2–4 business days
Glucose, FastingTest$24.99Yes1–3 business days
Lipid PanelTest · 4 biomarkers$24.99Yes1–3 business days
Cortisol (AM)Test$26.99No2–4 business days

Common questions

Do I need to fast for these tests?

Yes for fasting insulin, fasting glucose and the lipid panel (8–12 hours, water is fine). TSH, A1c and cortisol don't require fasting; draw cortisol before 9 a.m.

My TSH is normal — could my thyroid still be the cause?

Unlikely. A normal TSH makes hypothyroidism improbable as the cause of weight gain; the metabolic tests (insulin, A1c) more often explain it.

Educational content, not a diagnosis. Lab results are one input; a clinician interprets them with your history and exam.