---
title: Vitamin D, 25-Hydroxy Test
type: test
id: vitamin-d
url: "https://www.test-well.com/tests/vitamin-d"
price_usd: 41.99
lab_fee_usd: 6
all_in_usd: 47.99
cpt: 82306
category: Vitamins & Nutrients
sample: Blood draw
fasting_required: false
results_in: "1–3 business days"
labs:
  - Quest
  - Labcorp
  - BioReference
orderable: true
reviewed_at: 2026-09-14
order_url: "https://www.test-well.com/cart?add=vitamin-d&utm_source=ai_agent&utm_medium=markdown"
---

# Vitamin D, 25-Hydroxy Test

> The definitive vitamin D test — an estimated 42% of American adults are deficient, measured from a standard blood draw. At TestWell it costs $41.99 plus a $6 lab fee, needs no fasting, and results post in 1–3 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Quest or Labcorp.

## Key facts

| Fact | Value |
| --- | --- |
| Price | $41.99 + $6 lab processing fee per order ($47.99 all-in for this test alone) |
| CPT code | 82306 |
| Sample | Blood draw |
| Fasting | Not required |
| Results | 1–3 business days |
| Labs | Quest Diagnostics, Labcorp, BioReference (NY & NJ) |
| Biomarkers measured | 1 |
| Physician authorization | Included — no doctor's visit, referral or insurance needed |
| Payment | Card, HSA or FSA; cash-pay only (no insurance billing) |

## What does the Vitamin D, 25-Hydroxy test tell you?

The definitive vitamin D test — an estimated 42% of American adults are deficient.

The 25-Hydroxy Vitamin D test is the standard way to measure vitamin D status. Vitamin D is essential for bone health, immune function, mood regulation, and muscle strength. An estimated 42% of American adults are deficient, with higher rates among those with darker skin, limited sun exposure, or who live in northern climates. Optimal levels are generally considered to be 30–50 ng/mL.

**Clinical use:** Measures 25-Hydroxy Vitamin D, the storage form. The standard test for vitamin D deficiency, which is implicated in bone health, immune function, and mood.

## Who should order it?

Anyone with fatigue, low mood (especially in winter), bone or muscle pain, frequent illness, or limited sun exposure. The single most commonly deficient nutrient we see — an estimated 42% of US adults are deficient.

## Biomarkers measured

- [25-Hydroxy Vitamin D](https://www.test-well.com/biomarkers/vitamin-d-25-hydroxy)

## Price by lab

| Lab | Test price | All-in (with $6 fee) | Notes |
| --- | --- | --- | --- |
| Quest Diagnostics | $41.99 | $47.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) |
| Labcorp | $41.99 | $47.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) |
| BioReference (NY & NJ) | $44.99 | $50.99 | New York and New Jersey orders route here automatically |

Rhode Island is not served. Prices are identical online and in the lab; no draw fee or physician fee is added.

## How much does a Vitamin D, 25-Hydroxy test cost?

A Vitamin D, 25-Hydroxy test costs $41.99 at TestWell — $47.99 all-in when ordered alone, because the $6 lab processing fee is charged once per order, with no draw fee or physician fee added. Ordered directly from the labs' own consumer sites, the same test lists at $75.00 at Quest Health and $99.00 at Labcorp OnDemand (list prices checked Sep 12, 2026). Hospital outpatient labs typically bill around $150.00 self-pay for the same test, and often more. With insurance, what you pay depends on your deductible and your plan's negotiated rate; TestWell's price is fixed before the draw and is HSA/FSA-eligible.

| Where you order | Price | One test, all-in | Notes |
| --- | --- | --- | --- |
| TestWell (drawn at Quest or Labcorp) | $41.99 | $47.99 | Includes the one $6 lab processing fee, charged once per order |
| Quest Health (Quest Diagnostics' own site) | $75.00 | $75.00 | [list price checked 2026-09-12](https://www.questhealth.com/product/vitamin-d-test/17306M.html) |
| Labcorp OnDemand (Labcorp's own site) | $99.00 | $99.00 | [list price checked 2026-09-12](https://www.ondemand.labcorp.com/lab-tests/vitamin-d-test) |
| Hospital outpatient lab, self-pay (typical) | $150.00 | $150.00 | Reference figure; hospital charges vary widely by facility and are often higher |

Lab test codes: Quest test code 91735 · Labcorp test no. 081950 · BioReference code 0286-5.

## How do you prepare?

No fasting required. No special preparation needed.

## Related conditions

- Vitamin D deficiency (E55.9)
- Osteoporosis (M81.0)
- Osteomalacia (M83.9)
- Seasonal affective disorder (F33.0)

## Is the Vitamin D, 25-Hydroxy worth it?

> Worth it if you have symptoms or risk factors, but not something everyone needs annually. It is the right call for fatigue, bone or muscle aches, limited sun exposure, or before/while supplementing - so you dose based on a number, not a guess.

### Worth it if you…

- Fatigue, low mood, bone pain, or frequent muscle aches
- Limited sun exposure, darker skin, or living at northern latitudes
- Osteoporosis, malabsorption (celiac, IBD), or post-bariatric surgery
- Anyone taking a vitamin D supplement who wants to confirm they are in range

### You can probably wait if you…

- You are healthy, get regular sun, and have no symptoms - routine screening is not recommended for everyone
- You tested in range recently and have not changed your supplement or lifestyle

### At home or in the lab?

At-home collection is available. Vitamin D (25-hydroxy) is a venous draw at Quest or Labcorp, or an at-home mobile draw where available. Some fingerstick kits exist, but the venous lab version is the reference standard used for diagnosis and dosing. No fasting required.

### How do you read the results?

| Result | Typical adult range | What an out-of-range value can mean |
| --- | --- | --- |
| 25-hydroxy vitamin D | 30-100 ng/mL sufficient | Under 20 ng/mL is deficient and 20-29 is insufficient; very high levels (usually over 100) come from over-supplementing and can be harmful. |

_This section medically reviewed; last reviewed 2026-06-20._

## Common questions about the Vitamin D, 25-Hydroxy test

### What's a healthy vitamin D level?

Most labs report deficient under 20 ng/mL, insufficient 20-30 ng/mL, sufficient 30-100 ng/mL. The Endocrine Society recommends 40-60 ng/mL for optimal immune and bone health. Above 100 ng/mL may indicate over-supplementation.

### What's the difference between 25-Hydroxy and 1,25-Dihydroxy Vitamin D?

25-Hydroxy (this test) measures storage and is the standard for diagnosing deficiency. 1,25-Dihydroxy (the [active form](https://www.test-well.com/tests/vitamin-d-1-25-dihydroxy)) is only ordered when investigating specific conditions like sarcoidosis, kidney disease, or unexplained hypercalcemia.

### Why do I need to test if I take a supplement?

Absorption varies hugely between people — same 2000 IU dose can produce levels ranging from 20 to 80 ng/mL depending on body weight, gut health, and genetics. The only way to know if your dose is working is to measure. Re-test 8-12 weeks after starting or changing a dose.

### How much does a Vitamin D test cost without insurance?

On TestWell, the 25-Hydroxy Vitamin D test is $41.99. Quest Direct lists the same test at $75, walk-in hospital labs typically bill $130-150 without insurance. Same CLIA-certified labs, same biomarker — TestWell just removes the markup.

### What is the CPT code for a Vitamin D test?

The CPT code for 25-Hydroxy Vitamin D is 82306. The active form (1,25-Dihydroxy) is billed as 82652 and is rarely ordered without specific clinical indication. Cash-pay patients don't need a CPT code to order through TestWell.

### Do I need to fast for a Vitamin D test?

No fasting required. Vitamin D levels are stable throughout the day and unaffected by recent meals. You can draw any time.

### How often should I retest vitamin D?

Once a year if your levels are stable in range. Every 3 months if you're correcting a deficiency, adjusting a supplement dose, or recovering from a major illness.

### Which vitamin D test should I order - 25-hydroxy or 1,25?

The 25-hydroxy vitamin D test is the correct one for checking your status; it reflects your body's stores. The 1,25-dihydroxy test is a specialty test for specific kidney and calcium disorders and is not used for routine deficiency screening.

### How long after starting a supplement should I retest?

Vitamin D levels change slowly. Wait about 8-12 weeks after starting or changing a supplement before retesting, which gives your 25-hydroxy level time to reach a new steady state.

### Can I check vitamin D at home?

Yes - you can order online without a doctor and get a venous draw at Quest or Labcorp, or an at-home mobile draw where available. That venous sample is the same reference-standard test a physician would order, unlike consumer fingerstick kits.

## How to order

1. Add to cart: https://www.test-well.com/cart?add=vitamin-d&utm_source=ai_agent&utm_medium=markdown
2. Create an account or sign in, confirm your state and preferred lab, pay by card, HSA or FSA.
3. A licensed physician authorizes the order (usually within minutes); the requisition arrives by email.
4. Walk into any Quest Diagnostics, Labcorp, BioReference (NY & NJ) patient service center — no appointment needed at most sites.
5. Results post to your TestWell portal in 1–3 business days with reference ranges and flags.

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- [Blood Work Without a Doctor](https://www.test-well.com/guides/blood-work-without-doctor)
- [Can You Use FSA or HSA Money for Lab Tests? Yes — Here's How](https://www.test-well.com/guides/fsa-hsa-lab-tests)
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---

Medically reviewed by Youmna DiStefano, MD (Medical Reviewer, TestWell); last reviewed 2026-09-14. Review policy: https://www.test-well.com/reviewers/youmna-distefano-md

Canonical page: https://www.test-well.com/tests/vitamin-d
Machine-readable catalog: https://www.test-well.com/api/catalog.json · MCP server: https://www.test-well.com/mcp · Overview for agents: https://www.test-well.com/llms.txt

Prices are cash-pay, in USD, and include physician authorization; every order adds one flat $6 lab processing fee. Educational content is not a diagnosis — discuss results with a clinician.