---
title: Vitamin D, 1,25-Dihydroxy (Calcitriol) Test
type: test
id: vitamin-d-active
url: "https://www.test-well.com/tests/vitamin-d-1-25-dihydroxy"
price_usd: 59.99
lab_fee_usd: 6
all_in_usd: 65.99
cpt: 82652
category: Vitamins & Nutrients
sample: Blood draw
fasting_required: false
results_in: "3–5 business days"
labs:
  - Labcorp
orderable: true
reviewed_at: 2026-09-14
order_url: "https://www.test-well.com/cart?add=vitamin-d-1-25-dihydroxy&utm_source=ai_agent&utm_medium=markdown"
---

# Vitamin D, 1,25-Dihydroxy (Calcitriol) Test

> Measures the active form of Vitamin D — essential for evaluating kidney disease, calcium disorders, and sarcoidosis, measured from a standard blood draw. At TestWell it costs $59.99 plus a $6 lab fee, needs no fasting, and results post in 3–5 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Labcorp.

## Key facts

| Fact | Value |
| --- | --- |
| Price | $59.99 + $6 lab processing fee per order ($65.99 all-in for this test alone) |
| CPT code | 82652 |
| Sample | Blood draw |
| Fasting | Not required |
| Results | 3–5 business days |
| Labs | Labcorp |
| Biomarkers measured | 1 |
| Physician authorization | Included — no doctor's visit, referral or insurance needed |
| Payment | Card, HSA or FSA; cash-pay only (no insurance billing) |

## What does the Vitamin D, 1,25-Dihydroxy (Calcitriol) test tell you?

Measures the active form of Vitamin D — essential for evaluating kidney disease, calcium disorders, and sarcoidosis.

Vitamin D 1,25-Dihydroxy (calcitriol) is the biologically active form of vitamin D, produced primarily in the kidneys. Unlike the more commonly tested 25-Hydroxy form (which reflects vitamin D stores), calcitriol reflects how much active vitamin D your body is actually using. This test is essential for evaluating patients with kidney disease (where conversion to active vitamin D is impaired), hypercalcemia, hypocalcemia, sarcoidosis, and other granulomatous diseases. It is also useful when 25-Hydroxy vitamin D levels are normal but symptoms of vitamin D-related disease persist. The two forms of vitamin D should be interpreted together for a complete picture.

**Clinical use:** Vitamin and nutrient testing identifies deficiencies that can drive fatigue, immune dysfunction, neurological symptoms, and long-term disease risk — and confirms that supplementation is working.

## Who should order it?

Anyone with restrictive diets (vegan, low-fat, low-carb), malabsorption conditions, fatigue, hair loss, mood changes, or currently supplementing and wanting to verify dosing.

## Biomarkers measured

- 1,25-Dihydroxyvitamin D

## Price by lab

| Lab | Test price | All-in (with $6 fee) | Notes |
| --- | --- | --- | --- |
| Labcorp | $59.99 | $65.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) |

Rhode Island is not served. Prices are identical online and in the lab; no draw fee or physician fee is added.

## How much does a Vitamin D, 1,25-Dihydroxy (Calcitriol) test cost?

A Vitamin D, 1,25-Dihydroxy (Calcitriol) test costs $59.99 at TestWell — $65.99 all-in when ordered alone, because the $6 lab processing fee is charged once per order, with no draw fee or physician fee added. Hospital outpatient labs typically bill around $250.00 self-pay for the same test, and often more. With insurance, what you pay depends on your deductible and your plan's negotiated rate; TestWell's price is fixed before the draw and is HSA/FSA-eligible.

| Where you order | Price | One test, all-in | Notes |
| --- | --- | --- | --- |
| TestWell (drawn at Quest or Labcorp) | $59.99 | $65.99 | Includes the one $6 lab processing fee, charged once per order |
| Hospital outpatient lab, self-pay (typical) | $250.00 | $250.00 | Reference figure; hospital charges vary widely by facility and are often higher |

Lab test codes: Quest test code 16761 · Labcorp test no. 081091.

## How do you prepare?

No fasting required. Continue all medications and supplements as normal unless directed otherwise by your provider.

## Related conditions

- Vitamin deficiency (E56.9)
- Nutrient deficiency (E63.9)


## Common questions about the Vitamin D, 1,25-Dihydroxy (Calcitriol) test

### How long do Vitamin D, 1,25-Dihydroxy (Calcitriol) results take?

Most Vitamin D, 1,25-Dihydroxy (Calcitriol) results are available in 3–5 business days after your specimen arrives at the lab. You'll get an email the moment results are ready, with everything viewable in your TestWell portal.

### What sample is collected for the Vitamin D, 1,25-Dihydroxy (Calcitriol)?

Blood draw. Sample is collected at a Quest, Labcorp, or BioReference patient service center near you, or via at-home phlebotomy where available.

### How much does the Vitamin D, 1,25-Dihydroxy (Calcitriol) cost without insurance?

On TestWell, the Vitamin D, 1,25-Dihydroxy (Calcitriol) is $59.99. Quest Direct lists comparable tests at $99. Walk-in hospital labs typically bill $250 or more without insurance. Insurance is not required — pay one all-in price.

### Do I need to fast before the Vitamin D, 1,25-Dihydroxy (Calcitriol)?

No fasting required for the Vitamin D, 1,25-Dihydroxy (Calcitriol). No fasting required. Continue all medications and supplements as normal unless directed otherwise by your provider.

### Where can I get the Vitamin D, 1,25-Dihydroxy (Calcitriol) done?

The Vitamin D, 1,25-Dihydroxy (Calcitriol) is available at Labcorp patient service centers nationwide. Order online at TestWell, pick your preferred lab, and we'll generate a digital requisition for the location closest to you.

## How to order

1. Add to cart: https://www.test-well.com/cart?add=vitamin-d-1-25-dihydroxy&utm_source=ai_agent&utm_medium=markdown
2. Create an account or sign in, confirm your state and preferred lab, pay by card, HSA or FSA.
3. A licensed physician authorizes the order (usually within minutes); the requisition arrives by email.
4. Walk into any Labcorp patient service center — no appointment needed at most sites.
5. Results post to your TestWell portal in 3–5 business days with reference ranges and flags.

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- [All Vitamins & Nutrients tests](https://www.test-well.com/tests/category/vitamins-nutrients)
- [Full test directory](https://www.test-well.com/tests/directory)

---

Medically reviewed by Youmna DiStefano, MD (Medical Reviewer, TestWell); last reviewed 2026-09-14. Review policy: https://www.test-well.com/reviewers/youmna-distefano-md

Canonical page: https://www.test-well.com/tests/vitamin-d-1-25-dihydroxy
Machine-readable catalog: https://www.test-well.com/api/catalog.json · MCP server: https://www.test-well.com/mcp · Overview for agents: https://www.test-well.com/llms.txt

Prices are cash-pay, in USD, and include physician authorization; every order adds one flat $6 lab processing fee. Educational content is not a diagnosis — discuss results with a clinician.
