--- title: Thyroid Peroxidase (TPO) Antibodies Test type: test id: tpo-antibodies url: "https://www.test-well.com/tests/tpo-antibodies" price_usd: 25.99 lab_fee_usd: 6 all_in_usd: 31.99 cpt: 86376 category: Thyroid sample: Blood draw fasting_required: false results_in: "2–4 business days" labs: - Quest - Labcorp - BioReference orderable: true reviewed_at: 2026-09-14 order_url: "https://www.test-well.com/cart?add=tpo-antibodies&utm_source=ai_agent&utm_medium=markdown" --- # Thyroid Peroxidase (TPO) Antibodies Test > The most common test for Hashimoto's thyroiditis — measures antibodies that attack thyroid enzymes, measured from a standard blood draw. At TestWell it costs $25.99 plus a $6 lab fee, needs no fasting, and results post in 2–4 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Quest or Labcorp. ## Key facts | Fact | Value | | --- | --- | | Price | $25.99 + $6 lab processing fee per order ($31.99 all-in for this test alone) | | CPT code | 86376 | | Sample | Blood draw | | Fasting | Not required | | Results | 2–4 business days | | Labs | Quest Diagnostics, Labcorp, BioReference (NY & NJ) | | Biomarkers measured | 1 | | Physician authorization | Included — no doctor's visit, referral or insurance needed | | Payment | Card, HSA or FSA; cash-pay only (no insurance billing) | ## What does the Thyroid Peroxidase (TPO) Antibodies test tell you? The most common test for Hashimoto's thyroiditis — measures antibodies that attack thyroid enzymes. Thyroid Peroxidase (TPO) antibodies target an enzyme in the thyroid gland that plays a key role in producing thyroid hormones. Elevated TPO antibodies are the hallmark of Hashimoto's thyroiditis, the most common cause of hypothyroidism. They can be elevated years before thyroid hormone levels become abnormal. **Clinical use:** Thyroid Peroxidase antibodies — the most sensitive single marker for autoimmune thyroid disease (Hashimoto's). Used to confirm autoimmunity when TSH is abnormal. ## Who should order it? Anyone with subclinical or overt hypothyroidism, unexplained miscarriage or infertility, family history of autoimmune disease, or other autoimmune conditions (T1 diabetes, celiac, RA). ## Biomarkers measured - [TPO Antibodies](https://www.test-well.com/biomarkers/tpo-antibodies) ## Price by lab | Lab | Test price | All-in (with $6 fee) | Notes | | --- | --- | --- | --- | | Quest Diagnostics | $25.99 | $31.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) | | Labcorp | $26.99 | $32.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) | | BioReference (NY & NJ) | $29.99 | $35.99 | New York and New Jersey orders route here automatically | Rhode Island is not served. Prices are identical online and in the lab; no draw fee or physician fee is added. ## How much does a Thyroid Peroxidase (TPO) Antibodies test cost? A Thyroid Peroxidase (TPO) Antibodies test costs $25.99 at TestWell — $31.99 all-in when ordered alone, because the $6 lab processing fee is charged once per order, with no draw fee or physician fee added. Ordered directly from the labs' own consumer sites, the same test lists at $69.00 at Labcorp OnDemand (list prices checked Sep 12, 2026). Hospital outpatient labs typically bill around $150.00 self-pay for the same test, and often more. With insurance, what you pay depends on your deductible and your plan's negotiated rate; TestWell's price is fixed before the draw and is HSA/FSA-eligible. | Where you order | Price | One test, all-in | Notes | | --- | --- | --- | --- | | TestWell (drawn at Quest or Labcorp) | $25.99 | $31.99 | Includes the one $6 lab processing fee, charged once per order | | Labcorp OnDemand (Labcorp's own site) | $69.00 | $69.00 | [list price checked 2026-09-12](https://www.ondemand.labcorp.com/lab-tests/thyroid-peroxidase-tpo-antibody-test) | | Hospital outpatient lab, self-pay (typical) | $150.00 | $150.00 | Reference figure; hospital charges vary widely by facility and are often higher | Lab test codes: Quest test code 5081 · Labcorp test no. 006676 · BioReference code 0412-7. ## How do you prepare? No fasting required. No special preparation needed. ## Related conditions - Hashimoto thyroiditis (E06.3) - Postpartum thyroiditis (O90.5) - Subclinical hypothyroidism (E02) - Autoimmune thyroiditis (E06.3) ## Common questions about the Thyroid Peroxidase (TPO) Antibodies test ### What does a positive TPO antibody test mean? Levels above 35 IU/mL are typically considered positive. Combined with elevated TSH, it confirms Hashimoto's. Combined with normal TSH, it means euthyroid autoimmunity — you're at higher risk of developing hypothyroidism over the next 5-10 years and should be screened annually. ### Why test TPO if my TSH is normal? Hashimoto's antibodies often appear years before TSH abnormalities. Knowing you're TPO-positive lets you screen TSH more frequently, catch dysfunction early, and consider lifestyle changes (selenium, gluten reduction in celiac) that may slow progression. ### How much does a TPO antibody test cost without insurance? On TestWell, TPO antibodies cost $25.99. Quest Direct lists the same test at $50, walk-in hospital labs typically bill $100-150 without insurance. ### What is the CPT code for TPO antibodies? The CPT code for Thyroid Peroxidase Antibodies is 86376. ### Do I need to fast? No fasting required. TPO levels are stable across the day and unaffected by recent meals. ### Should I get TPO alone or with Thyroglobulin antibodies? TPO alone catches the vast majority of Hashimoto's cases. Adding Thyroglobulin antibodies (the [Thyroid Antibodies](https://www.test-well.com/tests/thyroid-antibodies) bundle) catches the ~5% of patients who are TPO-negative but Thyroglobulin-positive — worth it for a complete workup. ## How to order 1. Add to cart: https://www.test-well.com/cart?add=tpo-antibodies&utm_source=ai_agent&utm_medium=markdown 2. Create an account or sign in, confirm your state and preferred lab, pay by card, HSA or FSA. 3. A licensed physician authorizes the order (usually within minutes); the requisition arrives by email. 4. Walk into any Quest Diagnostics, Labcorp, BioReference (NY & NJ) patient service center — no appointment needed at most sites. 5. Results post to your TestWell portal in 2–4 business days with reference ranges and flags. ## Related - [Blood Tests for Thyroid Symptoms](https://www.test-well.com/guides/blood-tests-for-thyroid-symptoms) - [Blood Tests After Pregnancy](https://www.test-well.com/guides/blood-tests-after-pregnancy) - [Complete Thyroid Panel](https://www.test-well.com/tests/thyroid-complete-panel) — 4 tests · $109.99 - [Advanced Thyroid Panel](https://www.test-well.com/tests/advanced-thyroid-panel) — 7 tests · $129.99 - [Autoimmune Advanced Panel](https://www.test-well.com/tests/autoimmune-advanced-panel) — 11 tests · $159.99 - [Free T3 (Triiodothyronine)](https://www.test-well.com/tests/free-t3) — $30.99 - [Free T4 (Thyroxine)](https://www.test-well.com/tests/free-t4-test) — $23.99 - [Reverse T3](https://www.test-well.com/tests/reverse-t3) — $35.99 - [T3 Uptake](https://www.test-well.com/tests/t3-uptake-test) — $22.99 - [T3, Total (Triiodothyronine)](https://www.test-well.com/tests/total-t3-test) — $24.99 - [All Thyroid tests](https://www.test-well.com/tests/category/thyroid) - [Full test directory](https://www.test-well.com/tests/directory) --- Medically reviewed by Youmna DiStefano, MD (Medical Reviewer, TestWell); last reviewed 2026-09-14. Review policy: https://www.test-well.com/reviewers/youmna-distefano-md Canonical page: https://www.test-well.com/tests/tpo-antibodies Machine-readable catalog: https://www.test-well.com/api/catalog.json · MCP server: https://www.test-well.com/mcp · Overview for agents: https://www.test-well.com/llms.txt Prices are cash-pay, in USD, and include physician authorization; every order adds one flat $6 lab processing fee. Educational content is not a diagnosis — discuss results with a clinician.