--- title: Syphilis Antibody Test, Reverse Sequence Test type: test id: syphilis-reverse-sequence url: "https://www.test-well.com/tests/syphilis-antibody-reverse-sequence" price_usd: 49.99 lab_fee_usd: 6 all_in_usd: 55.99 cpt: 86780 category: STI & Infectious Disease sample: Blood draw fasting_required: false results_in: "2–5 business days" labs: - Quest orderable: true reviewed_at: 2026-10-12 order_url: "https://www.test-well.com/cart?add=syphilis-antibody-reverse-sequence&utm_source=ai_agent&utm_medium=markdown" --- # Syphilis Antibody Test, Reverse Sequence Test > Syphilis Antibody Test, Reverse Sequence measures Treponema pallidum Antibody (Total), RPR with Titer (reflex, if reactive) and Second Treponemal Test (reflex, if discordant) from a standard blood draw. At TestWell it costs $49.99 plus a $6 lab processing fee, needs no fasting, and results post in 2–5 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Quest. **On your lab requisition:** Quest "Syphilis Antibody Cascading Reflex" (test code 90349) ## Key facts | Fact | Value | | --- | --- | | Price | $49.99 + $6 lab processing fee per order ($55.99 all-in for this test alone) | | CPT code | 86780 | | Sample | Blood draw | | Fasting | Not required | | Results | 2–5 business days | | Labs | Quest Diagnostics | | Biomarkers measured | 3 | | Physician authorization | Included — no doctor's visit, referral or insurance needed | | Payment | Card, HSA or FSA; cash-pay only (no insurance billing) | ## What does the Syphilis Antibody Test, Reverse Sequence test tell you? The treponemal-first (reverse-sequence) syphilis algorithm — a total antibody screen with automatic reflex to RPR titer and a second confirmatory test. Quest test code 90349. This is the reverse-sequence syphilis algorithm, the approach many hospitals and laboratories now use for screening: the lab first runs a treponemal total-antibody immunoassay — antibodies to Treponema pallidum, which usually stay detectable for life after an infection. If that screen is reactive, the lab automatically reflexes to an RPR with titer, which indicates whether the infection is likely recent or active, and if the two results disagree, to a second, different treponemal test to settle it. Our standard Syphilis Test runs the traditional algorithm — an RPR screen first, with confirmatory testing if reactive — which is the right choice for most people who want a routine screen. Choose this reverse-sequence version if a clinician asked for a treponemal total antibody test or for Quest code 90349, if you have had treated syphilis in the past (the RPR can become non-reactive after treatment while treponemal antibodies persist), or if you want the algorithm that is more sensitive for very early and for late-stage infection. A reactive result needs follow-up with a clinician: because treponemal antibodies remain positive after successful treatment, the RPR titer and your history decide what it means. In New York and New Jersey, our standard Syphilis Test is already drawn at BioReference using its reverse algorithm. ## Biomarkers measured - Treponema pallidum Antibody (Total) - RPR with Titer (reflex, if reactive) - Second Treponemal Test (reflex, if discordant) ## Price by lab | Lab | Test price | All-in (with $6 fee) | Notes | | --- | --- | --- | --- | | Quest Diagnostics | $49.99 | $55.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) | Rhode Island is not served. Prices are identical online and in the lab; no draw fee or physician fee is added. ## How much does a Reverse Sequence Syphilis Antibody Test cost? A Reverse Sequence Syphilis Antibody Test costs $49.99 at TestWell — $55.99 all-in, since the $6 lab processing fee is charged once per order and there is no draw or physician fee. Hospital outpatient labs typically bill around $150.00 self-pay. With insurance you pay your plan's negotiated rate against your deductible; TestWell's price is fixed before the draw and HSA/FSA-eligible. | Where you order | Price | One test, all-in | Notes | | --- | --- | --- | --- | | TestWell (drawn at Quest) | $49.99 | $55.99 | Includes the one $6 lab processing fee, charged once per order | | Hospital outpatient lab, self-pay (typical) | $150.00 | $150.00 | Reference figure; hospital charges vary widely by facility and are often higher | Lab test codes: Quest test code 90349. ## How do you prepare? No fasting required. Treponemal antibodies typically become detectable 3–6 weeks after exposure; a negative result soon after a possible exposure should be repeated. ## Common questions about the Syphilis Antibody Test, Reverse Sequence test ### How long does it take to get Reverse Sequence Syphilis Antibody Test results? Reverse Sequence Syphilis Antibody Test results are typically ready in 2–5 business days after your draw at Quest. TestWell emails you the moment they post, and your results dashboard shows each marker against its reference range with a plain-English explanation. ### Do I need to fast, or can I eat before a Reverse Sequence Syphilis Antibody Test? No, fasting is not required for a Reverse Sequence Syphilis Antibody Test — you can eat normally and book any time of day. Treponemal antibodies typically become detectable 3–6 weeks after exposure; a negative result soon after a possible exposure should be repeated. ### Where can I get a Reverse Sequence Syphilis Antibody Test near me without a doctor? Order the Reverse Sequence Syphilis Antibody Test online from TestWell for $49.99 (plus a $6 per-order lab processing fee) — no doctor's visit, referral or insurance needed. A licensed physician authorizes the order, then you walk into any Quest patient service center near you for the draw (the lab locator on our site lists the closest ones by ZIP code), and results arrive in 2–5 business days. ### What is the CPT code for a Reverse Sequence Syphilis Antibody Test? The CPT code for a Reverse Sequence Syphilis Antibody Test is 86780. You can order it from TestWell for $49.99 — no doctor's visit or insurance required, with results in 2–5 business days. ### What is the Quest test code for a Reverse Sequence Syphilis Antibody Test? The Reverse Sequence Syphilis Antibody Test is Quest Diagnostics test code 90349 ("Syphilis Antibody Cascading Reflex") (CPT 86780). TestWell orders it under that code for $49.99 at Quest — no doctor's visit or insurance required, results in 2–5 business days. Codes verified against the labs' catalogs in 2026. ## How to order 1. Add to cart: https://www.test-well.com/cart?add=syphilis-antibody-reverse-sequence&utm_source=ai_agent&utm_medium=markdown 2. Create an account or sign in, confirm your state and preferred lab, pay by card, HSA or FSA. 3. A licensed physician authorizes the order (usually within minutes); the requisition arrives by email. 4. Walk into any Quest Diagnostics patient service center — no appointment needed at most sites. 5. Results post to your TestWell portal in 2–5 business days with reference ranges and flags. ## Related - [CMV IgG + IgM Antibodies (Cytomegalovirus)](https://www.test-well.com/tests/cmv-igg-igm-antibodies) — $74.99 - [CMV IgG Antibody (Cytomegalovirus)](https://www.test-well.com/tests/cmv-igg-antibody) — $40.99 - [CMV IgM Antibody (Cytomegalovirus)](https://www.test-well.com/tests/cmv-igm-antibody) — $40.99 - [Epstein-Barr Virus (EBV) Antibody Panel](https://www.test-well.com/tests/ebv-antibody-panel) — $94.99 - [Hepatitis B Test with Confirmation](https://www.test-well.com/tests/hepatitis-b-test) — $59.99 - [All STI & Infectious Disease tests](https://www.test-well.com/tests/category/sti-infectious-disease) - [Full test directory](https://www.test-well.com/tests/directory) --- Medically reviewed by Youmna DiStefano, MD (Medical Reviewer, TestWell); last reviewed 2026-10-12. Review policy: https://www.test-well.com/reviewers/youmna-distefano-md Canonical page: https://www.test-well.com/tests/syphilis-antibody-reverse-sequence Machine-readable catalog: https://www.test-well.com/api/catalog.json · MCP server: https://www.test-well.com/mcp · Overview for agents: https://www.test-well.com/llms.txt Prices are cash-pay, in USD, and include physician authorization; every order adds one flat $6 lab processing fee. Educational content is not a diagnosis — discuss results with a clinician.