--- title: Pregnenolone Test type: test id: pregnenolone url: "https://www.test-well.com/tests/pregnenolone" price_usd: 49.99 lab_fee_usd: 6 all_in_usd: 55.99 category: Hormones sample: Blood draw fasting_required: false results_in: "4–7 business days" labs: - Quest orderable: true order_url: "https://www.test-well.com/cart?add=pregnenolone&utm_source=ai_agent&utm_medium=markdown" --- # Pregnenolone Test > The 'mother hormone' made from cholesterol — the precursor to cortisol, DHEA, progesterone, testosterone and estrogen, measured from a standard blood draw. At TestWell it costs $49.99 plus a $6 lab processing fee, needs no fasting, and results post in 4–7 business days. Physician-authorized with no doctor's visit or insurance needed; draw at Quest. **On your lab requisition:** Quest "Pregnenolone, LC/MS" (test code 31493) ## Key facts | Fact | Value | | --- | --- | | Price | $49.99 + $6 lab processing fee per order ($55.99 all-in for this test alone) | | Sample | Blood draw | | Fasting | Not required | | Results | 4–7 business days | | Labs | Quest Diagnostics | | Biomarkers measured | 1 | | Physician authorization | Included — no doctor's visit, referral or insurance needed | | Payment | Card, HSA or FSA; cash-pay only (no insurance billing) | ## What does the Pregnenolone test tell you? The 'mother hormone' made from cholesterol — the precursor to cortisol, DHEA, progesterone, testosterone and estrogen. Pregnenolone sits at the top of the steroid hormone cascade: every adrenal and sex hormone is synthesized from it. Measured by LC/MS/MS, it is used by clinicians investigating low energy, low libido, mood and memory complaints, or unexplained low cortisol and DHEA-S, and by people on hormone optimization protocols who want to see the upstream picture rather than only the end products. Levels decline with age and can be suppressed by chronic stress or certain medications. Pregnenolone is interpreted together with DHEA-S, cortisol, progesterone and testosterone — a single value in isolation says little. ## Biomarkers measured - [Pregnenolone](https://www.test-well.com/biomarkers/pregnenolone) ## Price by lab | Lab | Test price | All-in (with $6 fee) | Notes | | --- | --- | --- | --- | | Quest Diagnostics | $49.99 | $55.99 | Choose at checkout; walk-in patient service centers nationwide (not NY/NJ) | Rhode Island is not served. Prices are identical online and in the lab; no draw fee or physician fee is added. ## How much does a Pregnenolone test cost? A Pregnenolone test costs $49.99 at TestWell — $55.99 all-in, since the $6 lab processing fee is charged once per order and there is no draw or physician fee. Hospital outpatient labs typically bill around $190.00 self-pay. With insurance you pay your plan's negotiated rate against your deductible; TestWell's price is fixed before the draw and HSA/FSA-eligible. | Where you order | Price | One test, all-in | Notes | | --- | --- | --- | --- | | TestWell (drawn at Quest) | $49.99 | $55.99 | Includes the one $6 lab processing fee, charged once per order | | Hospital outpatient lab, self-pay (typical) | $190.00 | $190.00 | Reference figure; hospital charges vary widely by facility and are often higher | Lab test codes: Quest test code 31493. ## How do you prepare? No fasting required. A morning draw is preferred; note any hormone or pregnenolone supplements for your provider. ## Common questions about the Pregnenolone test ### How long does it take to get Pregnenolone test results? Pregnenolone test results are typically ready in 4–7 business days after your draw at Quest. TestWell emails you the moment they post, and your results dashboard shows each marker against its reference range with a plain-English explanation. ### Do I need to fast, or can I eat before a Pregnenolone test? No, fasting is not required for a Pregnenolone test — you can eat normally and book any time of day. A morning draw is preferred; note any hormone or pregnenolone supplements for your provider. ### Where can I get a Pregnenolone test near me without a doctor? Order the Pregnenolone test online from TestWell for $49.99 (plus a $6 per-order lab processing fee) — no doctor's visit, referral or insurance needed. A licensed physician authorizes the order, then you walk into any Quest patient service center near you for the draw (the lab locator on our site lists the closest ones by ZIP code), and results arrive in 4–7 business days. ### What is the Quest test code for a Pregnenolone test? The Pregnenolone test is Quest Diagnostics test code 31493 ("Pregnenolone, LC/MS"). TestWell orders it under that code for $49.99 at Quest — no doctor's visit or insurance required, results in 4–7 business days. Codes verified against the labs' catalogs in 2026. ## How to order 1. Add to cart: https://www.test-well.com/cart?add=pregnenolone&utm_source=ai_agent&utm_medium=markdown 2. Create an account or sign in, confirm your state and preferred lab, pay by card, HSA or FSA. 3. A licensed physician authorizes the order (usually within minutes); the requisition arrives by email. 4. Walk into any Quest Diagnostics patient service center — no appointment needed at most sites. 5. Results post to your TestWell portal in 4–7 business days with reference ranges and flags. ## Related - [Men's Total Body Advanced Panel](https://www.test-well.com/tests/mens-total-body-advanced-panel) — 65 tests · $999.99 - [Women's Total Body Advanced Panel](https://www.test-well.com/tests/womens-total-body-advanced-panel) — 65 tests · $999.99 - [Adiponectin](https://www.test-well.com/tests/adiponectin) — $36.99 - [Aldosterone](https://www.test-well.com/tests/aldosterone-test) — $61.99 - [Antidiuretic Hormone (ADH) Profile](https://www.test-well.com/tests/antidiuretic-hormone-profile) — $109.99 - [Cortisol (AM)](https://www.test-well.com/tests/cortisol-am) — $26.99 - [Cortisol, PM](https://www.test-well.com/tests/cortisol-pm) — $34.99 - [All Hormones tests](https://www.test-well.com/tests/category/hormones) - [Full test directory](https://www.test-well.com/tests/directory) --- Canonical page: https://www.test-well.com/tests/pregnenolone Machine-readable catalog: https://www.test-well.com/api/catalog.json · MCP server: https://www.test-well.com/mcp · Overview for agents: https://www.test-well.com/llms.txt Prices are cash-pay, in USD, and include physician authorization; every order adds one flat $6 lab processing fee. Educational content is not a diagnosis — discuss results with a clinician.